BD, 339206, CD117 PE-Cy™7 104D2
100 Tests
Catalog Number: 339206
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Category
Description
The CD117 antibody, clone 104D2, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with the megakaryocytic cell line MOLM-1. The CD117 antibody binds to a 145-kilodalton (kDa) type I transmembrane glycoprotein in the receptor tyrosine kinase (RTK) family. The CD117 antigen is also known as c-kit and stem cell factor receptor (SCFR).
Specifications
General
| Verified Reactivity | Human |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. |
| Excitation Laser | Red Laser (633 nm) |
| Product Citations | Maas RJ, et al. 2020. Oncoimmunology. 9:1843247. PubMed Shemesh A, et al. 2022. J Exp Med. 219:. PubMed |
| RRID | AB_2565238 (BioLegend Cat. No. 339205) AB_2565239 (BioLegend Cat. No. 339206) |
| Structure | Type I transmembrane protein, Siglec family member, 75 kD |
| Distribution | NK cells, monocytes |
| Function | Inhibitory receptor of NK cells |
| Ligand/Receptor | Sialylated glycans (with α2,8 disialyl and α2,6 sialyl residues) |
| Cell Type | Monocytes, NK cells |
| Biology Area | Immunology |
| Molecular Family | CD Molecules, Siglec Molecules |
| Antigen References | Avril T, et al. 2006. Infection and Immunity 74:4133 Avril T, et al. 2004. J. Immunol. 173:6841 Yamaji T, et al. 2005. Glycobiology 15:667 |
| Gene ID | 27036 |
| UniProt | View information about CD328 on UniProt.org |
| Clone | 6-434 |
| Regulatory Status | RUO |
| Workshop | VIII 80652 |
| Other Names | Siglec7 (Sialic-binding Ig like lectin 7), p75, AIRM1 |
| Isotype | Mouse IgG1, κ |
