| Verified Reactivity | Human |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. |
| Excitation Laser | Red Laser (633 nm) |
| Application Notes | Clone 1412 can be used to stain CD20 after the cells have been treated with BioLegend's True-Phos™ Perm Buffer making it ideal for signaling pathway research and simultaneous detection of intracellular targets. |
| RRID | AB_2632885 (BioLegend Cat. No. 340516) |
| Structure | Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD |
| Distribution | B cell, T cell subsets |
| Function | B cell activation |
| Ligand/Receptor | Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82 |
| Cell Type | B cells, T cells |
| Biology Area | Costimulatory Molecules, Immunology |
| Molecular Family | CD Molecules |
| Antigen References | 1. Hultin L, et al. 1993. Cytometry 14:196. 2. Tedder T, et al. 1994. Immunol. Today 15:450. |
| Gene ID | 931 |
| UniProt | View information about CD20 on UniProt.org |
| Clone | 1412 |
| Regulatory Status | RUO |
| Other Names | B1, Bp35 |
| Isotype | Mouse IgG2a, κ |
