| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Cynomolgus, Rhesus |
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| Antibody Type | Monoclonal |
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| Host Species | Rat |
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| Immunogen | CX3CR1-EGFP fusion protein |
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| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
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| Preparation | The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions. |
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| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
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| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
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| Application | FC - Quality tested |
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| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm. |
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| Excitation Laser | Red Laser (633 nm) |
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| Application References(PubMed link indicates BioLegend citation) | Nishimura M, et al. 2002. J. Immunol. 168:6173. Nanki T, et al. 2002. Arthritis Rheum. 46:2878. Kobayashi T, et al. 2007. Inflamm. Bowel Dis. 13:837. Beziat V, et al. 2011. J. Immunol. 186:6753. PubMed. |
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| RRID | AB_2876645 (BioLegend Cat. No. 341631) AB_2876645 (BioLegend Cat. No. 341632) |
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| Structure | G-protein-coupled seven transmembrane receptor |
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| Distribution | NK cells, T cell subset, monocytes/macrophages, dendritic cells |
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| Function | cell adhesion and migration |
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| Ligand/Receptor | CX3CL1 (Fractalkine, FKN, neurotactin) |
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| Cell Type | Dendritic cells, Macrophages, Monocytes, NK cells, T cells |
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| Biology Area | Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | Cytokine/Chemokine Receptors, GPCR |
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| Antigen References | 1. Imai T, et al. 1997. Cell. 91:521. 2. Fong AM, et al. 1998. J. Exp. Med. 188:1413. 3. Auffray C, et al. 2009. J. Exp. Med. 206:595. |
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| Gene ID | 1524 |
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| UniProt | View information about CX3CR1 on UniProt.org |
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| Clone | 2A9-1 |
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| Regulatory Status | RUO |
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| Other Names | V28, GPR13, Chemokine C-X3-C receptor 1 |
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| Isotype | Rat IgG2b, κ |
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