| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus, Rhesus |
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| Antibody Type | Monoclonal |
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| Host Species | Rat |
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| Immunogen | C-fms transduced Kirsten strain murine sarcoma virus transformed NRK cells. |
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| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
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| Preparation | The antibody was purified by affinity chromatography. |
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| Concentration | 0.5 mg/ml |
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| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
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| Application | FC - Quality tested |
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| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes | It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1151. |
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| Application References(PubMed link indicates BioLegend citation) | Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed |
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| Product Citations | Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed |
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| RRID | AB_2085375 (BioLegend Cat. No. 347302) |
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| Structure | Single-pass type I membrane protein and is 150 kD. |
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| Distribution | Monocytes/macrophages, plasmacytoid and conventional dendritic cells, and osteoclasts. |
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| Ligand/Receptor | Macrophage Colony Stimulating Factor (M-CSF) and IL-34. |
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| Cell Type | Dendritic cells, Macrophages, Monocytes, Osteoclasts |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors |
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| Antigen References | 1. Sherr CJ, et al. 1989. Blood 73:1786 2. Roussel MF, et al. 1991. Nature 353:361. 3. Roussel MF, et al. 1989 P. Natl. Acad. Sci. USA 86:7924. |
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| Gene ID | 1436 |
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| UniProt | View information about CD115 on UniProt.org |
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| Clone | 9-4D2-1E4 |
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| Regulatory Status | RUO |
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| Workshop | V MA199 |
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| Other Names | M-CSFR,CSF-1R, FMS, CSF-1 Receptor |
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| Isotype | Rat IgG1, κ |
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