| Verified Reactivity | Human |
|---|
| Reported Reactivity | African Green, Baboon, Cynomolgus, Rhesus |
|---|
| Antibody Type | Monoclonal |
|---|
| Host Species | Rat |
|---|
| Immunogen | C-fms transduced Kirsten strain murine sarcoma virus transformed NRK cells. |
|---|
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA |
|---|
| Preparation | The antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer under optimal conditions. |
|---|
| Concentration | 0.5 mg/mL |
|---|
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
|---|
| Application | PG - Quality tested |
|---|
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-B antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. |
|---|
| Application Notes | It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1151. |
|---|
| Additional Product Notes | TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq. The barcode flanking sequences are GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNN (PCR handle), and NNNNNNNNNGCTTTAAGGCCGGTCCTAGC*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation. View more applications data for this product in our Scientific Poster Library. |
|---|
| Application References(PubMed link indicates BioLegend citation) | Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed |
|---|
| RRID | AB_2876658 (BioLegend Cat. No. 347327) |
|---|
| Structure | Single-pass type I membrane protein and is 150 kD. |
|---|
| Distribution | Monocytes/macrophages, plasmacytoid and conventional dendritic cells, and osteoclasts. |
|---|
| Ligand/Receptor | Macrophage Colony Stimulating Factor (M-CSF) and IL-34. |
|---|
| Cell Type | Dendritic cells, Macrophages, Monocytes, Osteoclasts |
|---|
| Biology Area | Immunology |
|---|
| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors |
|---|
| Antigen References | 1. Sherr CJ, et al. 1989. Blood 73:1786 2. Roussel MF, et al. 1991. Nature 353:361. 3. Roussel MF, et al. 1989 P. Natl. Acad. Sci. USA 86:7924. |
|---|
| Gene ID | 1436 |
|---|
| UniProt | View information about CD115 on UniProt.org |
|---|
| Clone | 9-4D2-1E4 |
|---|
| Regulatory Status | RUO |
|---|
| Workshop | V MA199 |
|---|
| Other Names | M-CSFR,CSF-1R, FMS, CSF-1 Receptor |
|---|
| Isotype | Rat IgG1, κ |
|---|
| Barcode Sequence | AATCACGGTCCTTGT |
|---|
