| Verified Reactivity | Human |
|---|---|
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA). |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions. |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. |
| Excitation Laser | Violet Laser (405 nm) |
| Application References(PubMed link indicates BioLegend citation) | Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules. New Jersey. (FC) |
| Product Citations | Wang X, et al. 2021. Cell Discovery. 7(1):60. PubMed Hu EY, et al. 2020. JCI Insight. 5:00. PubMed Schielke L, et al. 2022. Front Immunol. 13:916701. PubMed Moravcikova E, et al. 2018. Cytometry A. 93:894. PubMed Saygin C, et al. 2021. Leukemia. 35:3406. PubMed Verma M, et al. 2021. J Exp Med. 218:. PubMed |
| RRID | AB_2889063 (BioLegend Cat. No. 348805) |
| Distribution | T cells, B cells, NK cells, monocytes/macrophages, neutrophils |
| Cell Type | B cells, Dendritic cells, Macrophages, Mesenchymal Stem Cells, Monocytes, Neutrophils, NK cells, T cells |
| Biology Area | Immunology, Stem Cells |
| Molecular Family | CD Molecules |
| Gene ID | 91522149309314684929 |
| UniProt | View information about Lineage on UniProt.org |
| Clone | UCHT1; HCD14; 3G8; HIB19; 2H7; HCD56; |
| Regulatory Status | RUO |
| Other Names | Lin 1 |
| Isotype | Mouse IgG1, κ/Mouse IgG2b, κ |
