BD, 349502, Calibrite 2 Color Beads
25 Tests
Catalog Number: 349502
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| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human OCI-LY8 cell line |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Application | FC - Quality tested WB, IP - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Application Notes | Additional reported applications (for the relevant formats) include: Western Blotting3 and immunoprecipitation2,3. |
| Application References(PubMed link indicates BioLegend citation) |
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| Van Zelm MC, et al. 2010. J. Clin. Invest. 120:1265. Oren R, et al. 1990. Mol. Cell. Biol. 8:4007. (IP) Clark K, et al. 2004. J. Biol. Chem. 279(19):19401. (IP, WB) Mochida K, et al. 2008. J. Virol. 13:6711. Rappa G, et al. 2014. Mol Cancer Res. 12:1840. PubMed |
| Product Citations | Shi C, et al. 2019. FASEB J. 33:1617. PubMed Ciftci Dede E, et al. 2023. PLoS One. 18:e0282238. PubMed Mascarau R, et al. 2023. J Cell Biol. 222:. PubMed Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed Vrancx C, et al. 2021. Mol Neurobiol. 58:6647. PubMed Rodriguez L, et al. 2020. Cell Reports Medicine. 1(5):100078. PubMed Chetty VK, et al. 2022. Cancers (Basel). 14:. PubMed Desjardins P, et al. 2022. Int J Mol Sci. 23:. PubMed Porter RL, et al. 2022. J Clin Invest. :. PubMed van der Heide V, et al. 2022. Cell Rep. :110508. PubMed Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed Ter-Ovanesyan D, et al. 2021. Elife. 10:. PubMed Nakai W, et al. 2016. Sci Rep. 6: 33935. PubMed Araki Y, et al. 2021. Front Oncol. 11:667109. PubMed Lim K, et al. 2021. J Extracell Vesicles. 10:e12170. PubMed Zheng W, et al. 2022. Nat Biomed Eng. 6:979. PubMed Rappa G, et al. 2014. Mol Cancer Res. 12:1840. PubMed Bachurski D, et al. 2019. J Extracell Vesicles. 8:1596016. PubMed Lobastova L, et al. 2021. Frontiers in Cell and Developmental Biology. 9:698503. PubMed Shi C, et al. 2020. Biomedicines. 8:00. PubMed Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed You Y, et al. 2022. J Extracell Vesicles. 11:e12183. PubMed Menck K, et al. 2017. J Extracell Vesicles. 10.1080/20013078.2017.1340745. PubMed Dörsam B, et al. 2018. Front Immunol. 7:e34961. PubMed Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed |
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| RRID | AB_10642023 (BioLegend Cat. No. 349501) AB_10643417 (BioLegend Cat. No. 349502) |
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| Structure | 26 kD, type III transmembrane protein, member of the TM4SF tetraspanin family. Complexed with CD82, CD19, CD21, or CD4, CD8. |
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| Distribution | T cells, NK, monocytes, B cells, endothelial and epithelial cells, low on granulocytes. |
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| Function | Regulates cell activation and growth and cell aggregation. |
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| Cell Type | B cells, Endothelial cells, Epithelial cells, Monocytes, Neural Stem Cells, NK cells, T cells |
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| Biology Area | Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Transcription Factors |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | Van Zelm MC, et al. 2010. J. Clin. Invest. 120:1265. Fearon D, et al. 1995. Annu. Rev. Immunol. 13:127. Wright M, et al. 1994. Immunol. Today 15:588. |
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| Gene ID | 975 |
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| UniProt | View information about CD81 on UniProt.org |
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| Clone | 5A6 |
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| Regulatory Status | RUO |
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| Other Names | S5.7, CVID6, TSPAN28 |
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| Isotype | Mouse IgG1, κ |
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