Description
The G15-337 monoclonal antibody specifically recognizes rat IgG2b. It does not react with other Ig isotypes. A suspension of pooled rat Ig was used as the source of immunogen.
This antibody conjugate has been tested by ELISA (~2 μg/ml) to assure specificity and reactivity. For immunohistochemical staining of acetone-fixed frozen sections, a dilution range of 1:25 - 1:100 is suggested.
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
