Description
The OF-5A12 monoclonal antibody specifically recognizes the human variable alpha 7.2 region of the T cell receptor for antigen (TCR Vα7.2) that is joined with the Jα33 segment to form an invariant TCR which is characteristically expressed by mucosal-associated invariant T cells (MAIT cells). MAIT cell recognition of antigen is restricted by MHC-related molecule 1 (MR1), a nonpolymorphic Class Ib Major Histocompatibility Complex (MHC) molecule, that associates with β2-microglobulin. MAIT cells are found in human peripheral blood, mesenteric lymph nodes, liver, and mucosa associated with the lungs and intestines. MAIT cells play a role in detecting and fighting off microbial infections. The specificity of OF-5A12 for the TCR Vα7.2 was confirmed by its ability to block the binding of another human TCR Vα7.2-specific antibody, 3C10, to MAIT cells.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV480 under optimal conditions that minimize unconjugated dye and antibody.
1. This antibody was developed for use in flow cytometry.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Researchers should determine the optimal concentration of this reagent for their individual applications.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
9. BD Horizon Brilliant Violet 480 is covered by one or more of the following US patents: 8,575,303; 8,354,239.
