Description
The W23-251 monoclonal antibody specifically recognizes IL-17RA (CD217), which is also known as Interleukin-17 receptor A. IL-17RA (CD217) is a ~120 kDa single-pass type I transmembrane glycoprotein that is encoded by IL17RA. IL-17RA (CD217) belongs to the IL-17 receptor family which also includes IL-17 RB, IL-17RC, IL-17RD, and IL-17RE. IL-17RA (CD217) has a wide tissue distribution and is variably expressed on granulocytes, monocytes, macrophages, dendritic cells (DCs), T cells, B cells, NK cells, smooth muscle cells, endothelial cells, and epithelial cells. Interleukin-17A (IL-17A) or IL-17F homodimers, or IL-17A:IL-17F heterodimers can bind to and signal through functional IL-17 receptor complexes comprised of IL-17RA (CD217) and IL-17RC subunits. This leads to the cellular release of proinflammatory cytokines including IL-1, IL-6, and TNF, antimicrobial peptides, and chemokines that attract neutrophils. IL-17E/IL-25 binds to and signals through another functional IL-17 receptor complex comprised of IL-17RA (CD217) and IL-17RB subunits. IL-17E/IL-25 induces type-2 (Th2-like) immune responses and is implicated in asthma. IL-17C binds to IL-17 receptor complexes comprised of IL-17RA (CD217) and IL-17RE subunits and can stimulate the production of proinflammatory cytokines and antimicrobial peptides.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.
1. This antibody was developed for use in flow cytometry.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Researchers should determine the optimal concentration of this reagent for their individual applications.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
9. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
10. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
