Description
The 2F10 monoclonal antibody specifically recognizes exon v6-containing isoforms of the CD44 adhesion receptor (CD44v6). CD44 is a widely-expressed, 85-250 kDa type I transmembrane glycoprotein that regulates cellular adhesion, growth, and migration and is involved in cell-cell interactions. CD44 is a member of the hyaladherin family of hyaluronan-binding proteins. It serves as a multifunctional receptor for hyaluronic acid and other ligands including collagens, fibronectin, matrix metalloproteinases, and osteopontin. The standard form of CD44 (CD44s) contains an extracellular region with a hyaluronan-binding link domain and a stem region devoid of variable exon sequences that are followed by a transmembrane domain and a cytoplasmic tail. The cytoplasmic region is linked to the cytoskeleton and may be involved in signaling interactions. Alternative splicing of CD44 gene transcripts results in the differential inclusion of 10 variant exons (v1-10) within the stem region, either individually or in multiple combinations. This results in a large number of variant CD44 (CD44v) isoforms with different cell surface expression levels and functions. CD44 isoforms containing exon v6 (CD44v6) are variably expressed on epithelial cells, monocytes, macrophages, dendritic cells, and Langerhans cells. It is expressed on certain tumor cells and on cancer stem cells (CSCs) and is implicated in tumor cell metastasis. In addition to playing roles in cellular adhesion and migration, CD44 isoforms with exon v6 sequences may serve as coreceptors for some growth factors and cytokines including hepatocyte growth factor (HGF) which is involved in driving c-Met signaling.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.
1. This antibody was developed for use in flow cytometry.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Researchers should determine the optimal concentration of this reagent for their individual applications.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
9. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
10. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
