Description
The 159227 monoclonal antibody specifically recognizes MHC class I polypeptide-related sequence A (MICA) which is also known as MIC-A, MHC class I chain-related protein A, or stress inducible class I homolog. This ~70 kDa single-pass type I transmembrane glycoprotein is encoded by MICA (major histocompatibility complex class I chain-related gene A) which is a highly polymorphic gene. MICA is very homologous to another protein, MICB. Both MICA and MICB are homologous to major histocompatibility complex (MHC) class I molecules although they lack association with β2 microglobulin. MICA and MICB each contain three extracellular Ig domains but have no capacity to bind peptides. MICA is expressed on some gut epithelial cells. Its expression by other epithelial cells and cell types, including fibroblasts and endothelial cells, is induced by stress, eg, stress caused by bacterial and viral infections, autoimmunity, transplantation rejection, or cellular transformation. MICA and MICB are ligands for NKG2D (CD314), an activating receptor expressed by natural killer (NK) cells, γδ T cells, CD8+ αβ T cells, and some CD4+ αβ T cells.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.
1. This antibody was developed for use in flow cytometry.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Researchers should determine the optimal concentration of this reagent for their individual applications.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
9. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
10. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
