Description
The 134591 monoclonal antibody specifically recognizes CD159 antigen-like family member C (CD159c) which is also known as NKG2-C type II integral membrane protein or NKG2-C-activating NK receptor (NKG2C), or NK cell receptor C. CD159c (NKG2C) is a type II transmembrane glycoprotein that is encoded by KLRC2 (killer cell lectin like receptor C2) which belongs to the killer cell lectin-like receptor (KLR) family. CD159c (NKG2C) contains a C-type lectin domain in its extracellular region and is expressed by NK cells and some T cells. CD159c (NKG2C) can form a disulfide-bonded heterodimer with CD94 that non-covalently associates with DAP12 homodimers to form a functional signaling receptor complex. CD159c (NKG2C) binds to MHC class I HLA-E molecules on target cells to regulate NK cell activation.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV496 under optimal conditions that minimize unconjugated dye and antibody.
1. This antibody was developed for use in flow cytometry.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Researchers should determine the optimal concentration of this reagent for their individual applications.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
9. BD Horizon Brilliant Ultraviolet 496 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
