Description
The CMRF-56 monoclonal antibody specifically binds to a 96 kDa, early activation/differentiation antigen expressed on dendritic cells (DCs). The CMRF-56 antigen is expressed on isolated Langerhans cells, dermal DCs, migratory dermal DCs and monocyte derived DCs (GM-CSF, IL-4, TNF-α). Circulating blood DCs are CMRF-56-, but its expression is induced within 6 hrs of in vitro culture. Tonsil and synovial fluid DCs upregulate CMRF-56 expression following short-term in vitro culture. It is not detected on resting peripheral blood leukocytes, but is on a subpopulation of tonsil B cells and on in vitro cultured CD19+ cells. CMRF-56 was generated using the Hodgkin's-derived L428 cell line as immunogen. This reagent could be useful in flow cytometric or immunomagnetic separation procedures and immunohistochemical staining of acetone-fixed frozen tissue sections.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV661 under optimal conditions that minimize unconjugated dye and antibody.
1. This antibody was developed for use in flow cytometry.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Researchers should determine the optimal concentration of this reagent for their individual applications.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
9. BD Horizon Brilliant Ultraviolet 661 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
