Description
The 186107 monoclonal antibody specifically recognizes the Retinoic acid early inducible 1 (Rae-1) family of glycoproteins which is also known as retinoic acid early transcript 1. This family includes Rae-1α, β, δ, ε and γ, which are encoded by Raet1a, Raet1b, Raet1d, Raet1e, and Raet1c, respectively. Although expressed by some cells during mouse embryonic development, expression of these highly homologous membrane proteins is either low or absent on cells within adult tissues. These glycophosphatidylinositol (GPI)-linked proteins are structurally related to MHC class I molecules and contain alpha 1 and 2 domains. The Rae-1 family of glycoproteins serve as ligands for CD314 (NKG2D) which functions as an activating receptor expressed on some cytotoxic T cells and NK cells. Stress-induced Rae-1 proteins are expressed on tumor cells and might play a role in tumor rejection.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV661 under optimal conditions that minimize unconjugated dye and antibody.
1. This antibody was developed for use in flow cytometry.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Researchers should determine the optimal concentration of this reagent for their individual applications.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
9. BD Horizon Brilliant Ultraviolet 661 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
