| Source | Human TNFSF9, amino acids (Arg71-Glu254) (Accession# NP_003802), was expressed in 293E cells with a N-terminal His tag. |
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| Molecular Mass | The 193 amino acid recombinant protein has a predicted molecular mass of approximately 20.5 kD. The protein migrates at about 22 kD in DTT-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is His. |
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| Purity | >97%, as determined by Coomassie stained SDS-PAGE. |
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| Formulation | 0.22 µm filtered protein solution is in PBS, pH 7.2. |
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| Endotoxin Level | Less than 1.0 EU per µg of protein as determined by the LAL method. |
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| Concentration | 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
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| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | Human TNFSF9, when immobilized with anti-CD3 antibody, is able to inhibit IFN-γ secretion in human T cells in a dose-dependent manner with an ED50 of 0.4 - 4.0 µg/mL. Results may vary depending on human donors. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Monomer. |
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| Distribution | Constitutively expressed on a fraction of dendritic cells (DCs). Inducible on activated monocytes, macrophages, B cells, a small fraction of T cells, epithelial, and endothelial cells. |
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| Function | TNFSF9 plays roles in cytotoxic T cell activation, T cell proliferation, and apoptosis. |
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| Interaction | Activated T cells, natural killer cells (NK), macrophages, and dedritic cells. |
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| Ligand/Receptor | CD137. |
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| Bioactivity | TNFSF9 immobilized with anti-CD3 antibody is able to inhibit the secretion of IFN-γ by activated T cells. |
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| Biology Area | Immunology, Costimulatory Molecules |
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| Molecular Family | CD Molecules, Immune Checkpoint Receptors, Soluble Receptors |
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| Antigen References | 1. Vinay DS and Kwon BS. 1998. Semin. Immunol. 10:481. 2. Watts TH. 2005. Annu. Rev. Immunol. 23:23. 3. Pollok KE, et al. 1994. Eur. J. Immunol. 24:367. 4. Wang J, et al. 2010. J. Immunol. 185:7654. 5. Eun SY, et al. 2014. J. Immunol. 194:134. 6. Spencer AJ, et al. 2014. PLoS One 9:e105520. 7. Locatelli F. 2009. Blood 114:2208. |
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| Gene ID | 8744 |
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| UniProt | View information about TNFSF9 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | CD137L, 4-1BB-L, 4-1BBL, 41BBL, 4-1BB Ligand, 41BB Ligand |
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