| Source | Rat IL-3, amino acids Met-(Ile27-Cys169) (Accession# NM_031513.1), was expressed in E.coli. |
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| Molecular Mass | The 144 amino acid recombinant protein has a predicted molecular mass of approximately 16.3 kD. The protein migrates at approximately 18 kD in DTT-reducing conditions and 16 kD in non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Met. |
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| Purity | >95%, as determined by Coomassie stained SDS-PAGE. |
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| Formulation | 0.22 µm filtered protein solution is in PBS pH 7.2. |
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| Endotoxin Level | Less than 0.01 ng per µg cytokine as determined by the LAL method. |
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| Concentration | 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
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| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | ED50 = 1 - 5 ng/ml, corresponding to a specific activity of 0.2 — 1.0 x 106 units/mg, as determined by the dose dependent stimulation of a M-NFS-60 cell proliferation assay. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Cytokine. |
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| Distribution | Activated T cells, Th1, Th2, mast cells, eosinophils, keratinocytes, natural killer cells (NK), and endothelial cells. |
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| Function | IL-3 promotes both self-renewal and differentiation of early multipotential progenitors and is involved in inducible hematopoiesis in response to infections. IL-3 stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. |
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| Interaction | Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, and monocytic lineages. |
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| Ligand/Receptor | Heterodimer IL-3Rα (CD123) and two alternate β-subunits, AIC2A binds IL-3 and is specific to IL-3Rα, AIC2B (CDw131) shared in common with IL-5R, GM-CSFR. |
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| Cell Type | Hematopoietic stem and progenitors |
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| Biology Area | Cell Biology, Stem Cells |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Kohno M, et al. 2005. Blood 105:2059-2065. 2. Valent P and Dahinden CA. 2010. Curr. Opin. Hematol. 17:60-66. 3. Kleemann R, et al. 2008. Cardiovascular Research 79:360-376. 4. Murphy JM, Young IG. 2006. Vitam Horm. 74:1-30. 5. Luo XJ, et al. 2012. PLoS One 7:e50375. |
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| Gene ID | 24495 |
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| UniProt | View information about IL-3 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor |
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