Description
The C7 monoclonal antibody specifically binds to the low-density lipoprotein (LDL) receptor, a type I membrane protein that is encoded by the LDLR gene. LDL is the major cholesterol-carrying lipoprotein in plasma. Cell surface LDLR controls the level of cholesterol in plasma by binding to and internalizing LDL and transporting it to lysosomes where LDL is degraded, cholesterol is released into the cell, and LDLR is recycled back to the cell surface. Hence LDLR is found in cell-surface and intracellular membranes (eg, clathrin-coated pits, golgi, endosomes, and lysosomes). Expression of LDLR is a marker for in vitro differentiation of hepatocytes from human embryonic stem cells. LDLR is suspected to mediate infections by viruses that associate with lipoprotein in the blood. Mutations in LDLR are largely responsible for Familial Hypercholesterolemia (FH). The C7 monoclonal antibody has been reported to react with bovine and human LDLR, but not LDLRs of mouse, rat, Chinese hamster, rabbit or dog.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV496 under optimal conditions that minimize unconjugated dye and antibody.
1. This antibody was developed for use in flow cytometry.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Researchers should determine the optimal concentration of this reagent for their individual applications.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
9. BD Horizon Brilliant Ultraviolet 496 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
