Description
The OX-34 antibody reacts with CD2 (LFA-2), a member of the immunoglobulin superfamily. In the rat, CD2 is expressed on thymocytes, T lymphocytes in spleen and lymph node, dendritic epidermal T cells, splenic macrophages, and NK cells, but not on B cells, most intestinal intraepithelial lymphocytes, or peritoneal and liver macrophages. CD2 can associate with the T-cell receptor complex, and it may function in both intercellular adhesion and signal transduction. In the rat, CD48 and CD59 have been identified as ligands for CD2. OX-34 mAb binds to the extracellular portion of CD2, and it blocks the binding of CD2 to CD48. While OX-34 antibody does not activate T cells, it partially blocks activation by immobilized mAbs to CD3 (clone G4.18) and αβ T-cell receptor (clone R73), and it partially inhibits allogeneic mixed lymphocyte reactions. Moreover, in vivo administration of OX-34 antibody depletes peripheral T cells and prevents allograft rejection.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV496 under optimal conditions that minimize unconjugated dye and antibody.
1. This antibody was developed for use in flow cytometry.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Researchers should determine the optimal concentration of this reagent for their individual applications.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
9. BD Horizon Brilliant Ultraviolet 496 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
