The UCH-B1 (also known as, UCHB1) monoclonal antibody specifically recognizes the heavy chain constant region (Cµ) of human Immunoglobulin M (IgM) that is encoded by IGHM (immunoglobulin heavy constant mu). It does not crossreact with other immunoglobulin heavy or light chain isotypes. An intracytoplasmic form of IgM is expressed by pre-B cells whereas immature and a portion of mature B cells, including naive and memory B cells, express cell surface IgM. These forms of IgM can also be expressed by cells from some leukemias or lymphomas. Cell surface IgM is comprised of two type I transmembrane heavy chain glycoproteins (Igµ heavy chains) that are paired with immunoglobulin light chains of the same type, ie, either immunoglobulin kappa (Igκ) or lambda (Igλ) light chains. Cell surface IgM serves as a receptor that can specifically bind to antigens, including those expressed by microbial pathogens, and trigger the clonal expansion and differentiations of B cells into antibody-secreting plasma cells. A soluble pentameric form of IgM can be produced and secreted by plasma cells into the blood. Pentameric IgM is comprised of 5 monomers that consist of two Igµ heavy chains and two light chains that are complexed with a polypeptide J-chain. The UCH-B1 monoclonal antibody recognizes both cell surface and soluble human IgM. IgM is an important component in the first line of defense against foreign pathogens, but may also play a role in autoimmune diseases. The UCH-B1 antibody can reportedly activate or costimulate the proliferation of normal B cells or some transformed B cell lines.
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Researchers should determine the optimal concentration of this reagent for their individual applications.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. An isotype control should be used at the same concentration as the antibody of interest.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Alexa Fluor® is a registered trademark of Life Technologies Corporation.
6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
8. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email:
[email protected].
