Description
The 7C3.rMAb is a recombinant monoclonal antibody that specifically recognizes human CD15 which is a terminal carbohydrate epitope, 3-fucosyl-N-acetyllactosamine (3-FAL), on cell surface glycoproteins or glycolipids. CD15 is also known as Lewis X (LeX), X-Hapten, or Stage-Specific Embryonic Antigen-1 (SSEA-1). The 7C3.rMAb was derived from the 7C3 (also known as, PMN7C3) hybridoma. The 7C3 antibody was validated at HLDA V (Workshop Number MA88). The 7C3.rMAb has the same IgV-region heavy chain domain and Ig, κ light chain as the original mouse 7C3 IgG3, κ antibody. The remaining Ig constant heavy chain of 7C3.rMAb is derived from the mouse IgG1 heavy chain. CD15 is expressed on a variety of cell types including neutrophils, eosinophils, monocytes, macrophages, mast cells, and Langerhans cells. CD15 is also expressed on some epithelial cells, activated lymphocytes as well as tumor cells. CD15 is not expressed by platelets or erythrocytes. CD15 plays various roles in mediating cellular adhesion, activation, migration, and phagocytosis.
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application. For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
2. Researchers should determine the optimal concentration of this reagent for their individual applications.
3. An isotype control should be used at the same concentration as the antibody of interest.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
7. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
9. Alexa Fluor™ is a trademark of Life Technologies Corporation.
10. BD Horizon Brilliant Violet 650 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
