| Source | Human PTH, amino acids (Ser32-Gln115) (Accession# P01270), was expressed in E. coli. |
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| Molecular Mass | The 84 amino acid recombinant protein has a predicted molecular mass of approximately 9.4 kD. The protein migrates at approximately 13 kD in both DTT-reducing and non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Ser. |
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| Purity | >95%, as determined by Coomassie stained SDS-PAGE. |
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| Formulation | 0.22 µm filtered protein solution is in PBS, pH 7.2. |
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| Endotoxin Level | Less than 0.01 ng per µg cytokine as determined by the LAL method. |
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| Concentration | 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
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| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | ED50 = 10 - 60 ng/mL as measured by its ability to enhance BMP9 induced alkaline phosphatase production in MC3T3-E1 cells. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Additional Product Notes | View more applications data for this product in our Scientific Poster Library. |
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| Distribution | Parathyroid gland. |
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| Function | Regulation for calcium homeostasis. Its secretion is enhanced by low calcium levels and is inhibited by FGF-23. |
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| Interaction | Osteoblasts and osteocytes. |
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| Ligand/Receptor | PTH1R and PTH2R. |
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| Bioactivity | PTH enhances BMP9 induced alkaline phosphatase production in MC3T3-E1 cells. |
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| Cell Type | Mesenchymal Stem Cells |
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| Biology Area | Cell Biology, Stem Cells |
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| Molecular Family | Cytokines/Chemokines, Growth Factors |
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| Antigen References | 1. Goswami R, et al. 2004. J. Clin. Endocr. Metab. 89:4840-4845. 2. Lim SK, et al. 1992. Endocrinology 131:2325-2330. 3. Brommage R, et al. 1999. J. Clin. Endocr. Metab. 84:3757-3763. 4. Scillitani A, et al. 2011. J. Endocrinol. Invest. 34:23-6. 5. Ben-Dov IZ, et al. 2007. J. Clin. Invest. 117:4003-8. 6. Liu Z, et al. 2010. PLoS Genet. 6:e1001251. 7. Lombardi G, et al. 2010. J. Endocrinol. Invest. 33:22-6. 8. Dobnig H. 2004. Expert Opin. Pharmacother. 5:1153-62. 9. Vaidya A, et al. 2015. J. Clin. Endocrinol. Metab. 6:2396-404. |
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| Gene ID | 5741 |
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| UniProt | View information about PTH on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Parathyrin, Parathormone |
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