Description
The WTH1 monoclonal antibody specifically binds to rat CD1d and crossreacts with mouse CD1d (with stronger reactivity against mouse CD1d1 than CD1d2). CD1d is a type I transmembrane glycoprotein that is noncovalently associated with β2-microglobulin. CD1d is expressed on various cell types including subsets of thymocytes, T cells, B cells, monocytes, macrophages, dendritic cells, endothelial cells and epithelial cells. Although structurally similar to MHC Class I antigens, CD1d molecules are rather non-polymorphic and serve to present non-peptide antigens such as endogenous or microbial glycolipids to T lymphocytes (NKT cells). The WTH2 monoclonal antibody reportedly binds to a non-overlapping epitope on rat CD1d and also crossreacts with mouse CD1d when compared with WTH1. Both WTH1 and WTH2 reportedly interfere with antigen recognition by CD1d-restricted T cells.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Researchers should determine the optimal concentration of this reagent for their individual applications.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. CF™ is a trademark of Biotium, Inc.
7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
