BD, 753502, RY586 Rat Anti-Mouse CD184 2B11/CXCR4
50 µg
Catalog Number: 753502
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Description
The 2B11/CXCR4 monoclonal antibody specifically binds to mouse CD184, which is also known as the C-X-C Chemokine Receptor type 4 , CXCR4. CXCR4 (previously known as Fusin and LESTR), is a seven-transmembrane, G-protein-coupled receptor. It is the specific receptor for the CXC chemokine, SDF-1/CXCL12. Mouse CXCR4 shows 91% homology at the amino acid level with human CXCR4. CXCR4 is widely expressed by hematopoietic and non-hematopoietic cell types including neutrophils, monocytes, T cells, B cells, CD34-positive progenitor cells, endothelial cells, neurons and astrocytes. Human CXCR4 is used by T-tropic HIV-1 as a co-receptor for viral entry. The mouse Cxcr4 gene has been mapped to chromosome 1.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Researchers should determine the optimal concentration of this reagent for their individual applications.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. CF™ is a trademark of Biotium, Inc.
7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Researchers should determine the optimal concentration of this reagent for their individual applications.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. CF™ is a trademark of Biotium, Inc.
7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Specifications
General
| Source | Human SHH, amino acids (Cys24-Gly197) (Cys24Ile-Ile) (Accession# NP_000184), with a N-terminal Met was expressed in E. coli. |
|---|---|
| Molecular Mass | The 176 amino acid recombinant protein has a predicted molecular mass of approximately 19.8 kD. The protein migrates at approximately 22 kD in both DTT-reducing and non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Met. |
| Purity | >95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in 10 mM sodium phosphate buffer, 0.3M NaCl, and in pH 7.2 |
| Endotoxin Level | Less than 0.01 ng per µg cytokine as determined by the LAL method. |
| Concentration | 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | ED50 = 0.15 - 0.6 µg/mL as measured by its ability to induce C3H10T1/2 cell differentiation in the presence of hBMP9 (20 ng/mL) as measured by alkaline phosphatase production. |
| Application | Bioassay |
| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
| Additional Product Notes | View more applications data for this product in our Scientific Poster Library. |
| Product Citations | Huang WK, et al. 2021. Cell Stem Cell. 28:1657. PubMed |
| Distribution | Notochord, fetal intestine, liver, lung, and kidney. |
| Function | SHH is essential in the patterning of early embryo, differentiation of the gastric epithelium, and T cells. |
| Interaction | Ventral neural tube, ventral somites, limb axis in developing embryo, and hematopoietic stem cells. |
| Ligand/Receptor | Patched-1 (PCTH1), patched 2 (PCTH2), and smoothened (SMO). |
| Bioactivity | SHH enhances BMP9 induced differentiation of C3H10T1/2 cells. |
| Biology Area | Cell Biology, Neuroscience, Stem Cells, Synaptic Biology |
| Molecular Family | Cytokines/Chemokines, Growth Factors |
| Antigen References | 1. Echelard Y, et al. 1993. Cell 75:1417-1430. 2. Marigo V, et al. 1995. Genomics 28:44-51. 3. Porter JA, et al. 1996. Science 5285:255-9. 4. Zeng X, et al. 2001. Nature 411:716-720. 5. Johnson RL, et al. 1997. Cell 90:979-990. 6. Briscoe J, Therond PP. 2013. Nat. Rev. Mol. Cell Biol. 14:416-29. 7. Filmus J, Capurro M. 2014. Matrix Biol. 35:248-52. 8. Chuang PT, McMahon AP. 1999. Nature 397:617-21. 9. Crompton T, et al. 2007. Nat. Rev. Immunol. 7:726-35. 10. Shiotani A, et al. 2005. Am. J. Gastroenterol. 100:581-7. |
| Gene ID | 6469 |
| UniProt | View information about Sonic Hedgehog on UniProt.org |
| Regulatory Status | RUO |
| Other Names | SHH, TPT, HHG1, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5 |
| GMP Version Available | View Product |
