Description
The 512 monoclonal antibody specifically recognizes a 65-kDa and 55-kDa molecules of the erythrocyte membrane, which are the rat counterpart of mouse Crry/p65, a membrane inhibitor of complement component C3 convertase. Crry/p65 is widely distributed, predominantly expressed on endothelial cells and all circulating cells. This molecule was also detected on immature hepatocytes, systemic endothelial cells, skin fibroblasts, bronchial epithelial cells, bile canaliculi, the Schwann sheath of peripheral nerve fibers, and ependymal cells. Mouse Crry/p65 uses the same mechanisms as human Decay-Accelerating Factor (DAF) and human Membrane Cofactor Protein (MCP) in inhibiting C3 and C5 convertases, but it is not homologous to either DAF or MCP. It has been proposed that Crry/p65 is the mouse genetic homologue of human CR1 (CD35).
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Researchers should determine the optimal concentration of this reagent for their individual applications.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. CF™ is a trademark of Biotium, Inc.
7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
