Description
The 705518 monoclonal antibody specifically recognizes C-type lectin domain family 2 member A (CLEC-2A) which is also known as Proliferation-induced lymphocyte-associated receptor (PILAR) or Keratinocyte-associated C-type lectin (KACL). CLEC-2A is a ~35 kDa type II transmembrane glycoprotein that is encoded by CLEC2A which belongs to the C-type lectin superfamily. The extracellular region of CLEC-2A contains one C-type lectin domain. CLEC-2A (PILAR) is expressed on keratinocytes and can activate NK cells through binding to their surface KLRF2 molecules. It is also expressed on activated CD8+ T cells and can costimulate proliferative responses upon interaction with CD161.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
