Description
The ASC-8 monoclonal antibody specifically recognizes CD104 which is also known as Integrin beta 4 (Integrin β4 or β4 Integrin). Integrins mediate important intercellular and cell-extracellular matrix interactions which are essential for cellular adhesion and migration as well as for regulating cellular growth and survival. CD104 (Integrin β4) is an ~205 kDa single-pass type I transmembrane glycoprotein that is encoded by ITGB4 (Integrin subunit beta 4). CD104 (Integrin β4) noncovalently associates with CD49f (Integrin α6 chain) to form the Integrin α6/β4 (CD49f/CD104) complex. CD104 (Integrin β4) is expressed on basal epithelial cells, Schwann cells, and some endothelial cells and tumor cells. The CD49f/CD104 complex serves as an adhesion receptor that binds to laminins and keratin filaments. It is a component of epidermal hemidesmosomes that facilitate the stable adhesion of basal epithelial cells to the underlying basement membrane.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Researchers should determine the optimal concentration of this reagent for their individual applications.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. CF™ is a trademark of Biotium, Inc.
7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
10. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
