The PS/2 monoclonal antibody specifically recognizes Mouse CD49d which is also known as Integrin alpha 4 (Integrin α4 or α4 Integrin), Very Late Activation protein 4 receptor alpha 4 subunit (VLA-4 alpha or VLA-4α), or Lymphocyte Peyer patch adhesion molecules subunit alpha (LPAM alpha). CD49d is an ~150 kDa single-pass type I transmembrane glycoprotein that is encoded by Itga4. CD49d is widely expressed on thymocytes, T cells, B cells, monocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells (DC), and some non-hematopoietic cells, but not on neutrophils, platelets, or red blood cells. CD49d serves as the alpha subunit in heterodimeric receptors for some extracellular matrix components and cell-associated ligands. CD49d associates with Integrin beta 1 (CD29) to form the CD49d:CD29 (VLA-4) complex that binds to either fibronectin or VCAM-1 (CD106). CD49d heterodimerizes with Integrin beta 7 to form Integrin alpha4:beta7 (α4β7 Integrin or LPAM-1) that binds to the Mucosal addressin cell adhesion molecule 1 (MAdCAM-1). The PS/2 antibody has been used as a blocking antibody in functional studies that determined these receptors can play important roles in lymphopoiesis, leucocyte migration, and T cell activation. The PS/2 antibody reportedly crossreacts with Human CD49d and partially shares an epitope with the R1-2 monoclonal antibody that specifically recognizes Mouse CD49d.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application. For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
2. Researchers should determine the optimal concentration of this reagent for their individual applications.
3. An isotype control should be used at the same concentration as the antibody of interest.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
7. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
9. Alexa Fluor™ is a trademark of Life Technologies Corporation.
10. BD Horizon Brilliant Violet 711 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
11. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
