BD, 756202, RB545 Mouse Anti-Rat CD71 OX-26
50 µg
Catalog Number: 756202
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Description
The OX-26 monoclonal antibody specifically recognizes the transferrin receptor which is also known as CD71. CD71 is expressed on proliferating cells and brain endothelium. The OX-26 antibody does not block transferrin binding to its receptor.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
5. An isotype control should be used at the same concentration as the antibody of interest.
6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
7. Researchers should determine the optimal concentration of this reagent for their individual applications.
8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
5. An isotype control should be used at the same concentration as the antibody of interest.
6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
7. Researchers should determine the optimal concentration of this reagent for their individual applications.
8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Specifications
General
| Source | Human Cystatin C, amino acids (Ser27-Ala146) (Accession# P01034), was expressed in 293E cells. The carboxi terminal contains a TG-10His-GGQ-tag. |
|---|---|
| Molecular Mass | The 135 amino acid recombinant protein has a predicted molecular mass of approximately 15.1 kD. The DTT-reducing and non-reducing protein migrates at approximately 17 kD by SDS-PAGE. The predicted N-terminal amino acid is Ser. |
| Purity | >95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein is in 20 mM HEPES, 0.1 M NaCl, and in pH 7.2. |
| Endotoxin Level | Less than 0.1 EU per µg cytokine as determined by the LAL method. |
| Concentration | 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | IC50 < 6.0 nM, as determined by inhibiting human Papain (0.05 µg/mL) activity when 100 uM of Z-FR-AMC is used as a papain peptide substrate. |
| Application | Bioassay |
| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
| Product Citations | Anchan A, et al. 2020. Int J Mol Sci. :21. PubMed |
| Structure | Monomer. |
| Distribution | Considered ubiquitously expressed, biological fluids, dendritic cells, and macrophages. |
| Function | Inhibitor of cysteine proteases (capthepsins B, H, K, L, and S). Transcription factor IFN regulatory factor 8 (IRF-8) induces cystatin C in mouse primary dendritic cells. IL-10 inhibits IRF-8 and consequently Cystatin C. |
| Interaction | Normal and cancer cells. |
| Ligand/Receptor | Papain-like cysteine proteases, cathepsins B, H, K, L, and S, and legumain. |
| Bioactivity | Inhibits cystein proteases. |
| Biology Area | Cancer Biomarkers, Cell Biology |
| Molecular Family | Enzymes and Regulators |
| Antigen References | 1. Grubb A, et al. 1982. Proc. Nat. Acad. Sci. 79:3024. 2. Janowski R, et al. 2001. Nat. Struct. Biol. 8:316. 3. Sokol JP, Schiemann WP. 2004. Mol. Cancer Res. 2:183. 4. Bird PI, et al. 2009. Nat Rev Immunol. 9:871. 5. Kaur G, Levy E. 2012. Front Mol. Neurosci. 5:79. 6. Xu Y, et al. 2014. J Biol Chem. 289:9730. 7. Xu Y, et al. 2015. Immunol. Cell Biol. 5:442-51. |
| Gene ID | 1471 |
| UniProt | View information about Cystatin C on UniProt.org |
| Regulatory Status | RUO |
| Other Names | Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin, gamma-CSF, post-gamma protein |
| GMP Version Available | View Product |
