Description
The MAC2-158 monoclonal antibody (also known as Clone MAC 2-158) specifically binds to human CD163. CD163 is also known as Scavenger receptor cysteine-rich type 1 protein M130 (M130), SCARI1 (SR-I1), or Hemoglobin scavenger receptor (HbSR). CD163 is an ~130 kDa type I transmembrane glycoprotein comprised of an extracellular domain with nine cysteine-rich (SRCR) scavenger receptor class B domains followed by a transmembrane region and a short cytoplasmic tail. CD163 is expressed on most peripheral blood monocytes, tissue macrophages, and a subset of dendritic cells. CD163 serves as a high affinity receptor for hemoglobin and haptoglobin and mediates endocytosis of hemoglobin and haptoglobin complexes by macrophages. This scavenging function may protect tissues from hemoglobin-mediated oxidative damage and contribute to the uptake and recycling of iron. CD163 can also reportedly bind to (TNF-a)-like weak inducer of the apoptosis (TWEAK) protein and some pathogenic bacteria. A cleaved, soluble form of CD163 can reportedly play an anti-inflammatory role and serve as a marker for macrophage activation in inflammatory responses. High-affinity binding of the MAC2-158 antibody to CD163 is reportedly unaffected by extracellular calcium levels. This clone can be used to measure CD163 expression in freshly drawn whole blood samples stabilized with commonly used anticoagulants, eg, EDTA, citrate or heparin.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
5. An isotype control should be used at the same concentration as the antibody of interest.
6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
7. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
8. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
9. Researchers should determine the optimal concentration of this reagent for their individual applications.
10. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
