Description
The A2MR-α2 monoclonal antibody specifically reacts with a 600 kDa, type I membrane single protein, also known as the α2 Macroglobulin (α2M) receptor/low density lipoprotein receptor-related protein 1 (LRP-1). Reported to be an endocytic receptor involved with intracellular signalling, lipid homeostasis, clearance of apoptotic cells, and α2 Macroglobulin mediated clearance of secreted amyloid precursor protein found in Alzheimer patients. The single chain receptor undergoes cleavage, shortly after synthesis, into the 85 kDa transmembrane β chain that non-covalently binds to the extracellular 500-515 kDa a chain. It has a broad cellular distribution, but in the hematopoietic system it is expressed on monocyte lineage cells. α2M/LRP-1 mediates endocytosis of a variety of ligands including α2M-proteinase complexes, plasminogen activators in complex with plasminogen activator inhibitor, or Pseudomonas Exotoxin A. Ligand binding to α2M/LRP-1 is followed by rapid transport of the ligand to lysosomes for degradation.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
5. An isotype control should be used at the same concentration as the antibody of interest.
6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
7. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
8. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
9. Researchers should determine the optimal concentration of this reagent for their individual applications.
10. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
