Description
The YTS156.7.7.rMAb is a recombinant monoclonal antibody derived from YTS156.7.7 hybridoma cells that specifically recognizes the β chain of the CD8 differentiation antigen (CD8b), which is also known as Lymphocyte antigen 3 (Ly-3) or Lyt-3. CD8b is a single-pass type I transmembrane glycoprotein that is encoded by Cd8b1 (CD8 antigen, beta chain 1) which belongs to the Ig superfamily. CD8b is comprised of an N-terminal IgV domain in its extracellular region followed by a transmembrane region and a cytoplasmic tail. CD8b can bind to the CD8 α chain (CD8a) to form the disulfide-linked CD8 αβ (CD8ab) heterodimer also known as Ly2,3 or Lyt-2,3. This heterodimer is expressed on most thymocytes and a subpopulation of MHC class I-restricted mature T cells. CD8 αβ functions as an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and the protein tyrosine kinase, lck (p56lck).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
5. An isotype control should be used at the same concentration as the antibody of interest.
6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
7. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
8. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
9. Researchers should determine the optimal concentration of this reagent for their individual applications.
