Description
The 587702 monoclonal antibody specifically recognizes the alternately-spliced variant 2 of CD97 (CD97 v2). CD97 is an N-glycosylated seven-span transmembrane protein that is encoded by Adgre5 (Adhesion G protein-coupled receptor E5) which belongs to the EGF-TM7 subfamily within the G-protein coupled receptor (GPCR) II family. Several CD97 isoforms have been described that have either three or four epidermal growth factor-like (EGF) domains in their N-terminal extracellular region. Compared with the longest form, CD97 v2 has a 94-amino acid deletion that results in the loss of the third EGF-like repeat. CD97 is expressed on monocytes, macrophages, T cells, B cell subsets, dendritic cells, granulocytes, hematopoietic stem cells and progenitor cells, and smooth muscle cells. Through its EGF domains, CD97 binds to glycophosphatidylinositol-linked CD55 that is also known as decay accelerating factor (DAF) and is involved in the regulation of complement activity. CD97 plays a role in angiogenesis, neutrophil migration, and host defense.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
6. An isotype control should be used at the same concentration as the antibody of interest.
7. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
8. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
9. Researchers should determine the optimal concentration of this reagent for their individual applications.
