Description
The 4H9 (Leu-9) monoclonal antibody specifically recognizes human CD7 which is also known as T-cell leukemia antigen, T-cell surface antigen Leu-9, and LEU-9. CD7 is a ~40 kDa type I transmembrane glycoprotein that has an extracellular region with an N-terminal IgV-like domain followed by an extended O-glycosylated stalk region, a transmembrane region and cytoplasmic tail. The CD7 antigen is expressed throughout T-lymphocyte differentiation. It is present on 85% to 90% of peripheral blood T lymphocytes. In normal individuals, CD7 is expressed on all CD8+ lymphocytes, approximately 90% of CD4+ lymphocytes, and most NK cells. CD7 is weakly expressed on monocytes but not on granulocytes or B lymphocytes. It is expressed on hematopoietic progenitors and 50% of thymocytes. In leukemias, the CD7 antigen is present on the majority of T-lymphoid lineages. CD7 may function in cellular adhesion and play a role in interactions between T cells as well as T cells and B cells.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
5. An isotype control should be used at the same concentration as the antibody of interest.
6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
7. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
8. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
9. Researchers should determine the optimal concentration of this reagent for their individual applications.
10. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
