Description
The KJ16-133 monoclonal antibody specifically recognizes Mouse T cell receptors (TCR) containing β-chains with TCR Vβ8.1 or TCR Vβ8.2 regions that belong to TCR Vβ8 family which also includes TCR Vβ8.3. TCR Vβ8.1 and TCR Vβ8.2 (TCR Vβ8.1, 8.2) are alternatively expressed by subpopulations of mouse thymocytes and peripheral CD4+ and CD8+ T cells in most mouse strains including BALB/c, C57BL6, B10.A, and AKR. The Tcrb-V8 subfamily gene loci are deleted in mice having the a (eg, C57BR, C57L, SJL, SWR) or c (eg, RIII) MHC haplotype. Mouse mammary tumor viruses (MMTV) exist in two forms as either germline integrants in the mouse genome (Mtv) or as infectious virus particles that can affect the size of TCR Vβ 8+ T cell subpopulations. TCR Vβ 8.1+ T cells are clonally eliminated in mice expressing superantigen encoded by the Mtv-7 provirus (eg, AKR, CBA/J, C58, DBA/2), and activation or elimination of TCR Vβ 8.1+ T cells by this determinant is partially dependent upon presentation by I-E. Mtv-43, Mtv-44, and/or exogenous MMTV-SW superantigens also cause incomplete elimination of TCR Vβ 8.1+ T cells. Likewise, staphylococcal enterotoxin B (SEB), in association with antigen presenting cells expressing I-A and/or I-E, stimulates TCR Vβ 8.1+ T cells and selectively eliminates those T cells in vivo. In addition to expression on conventional T lymphocytes, Vβ8.2 is the predominant TCR β chain variable region expressed on NK-T cells.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
3. For U.S. patents that may apply, see bd.com/patents.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
7. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
9. An isotype control should be used at the same concentration as the antibody of interest.
10. CF™ is a trademark of Biotium, Inc.
