RFQ
Menu
BD

BD, 759709, RY610 Rat Anti-Mouse ENPP1 YE1/19.1

50 µg
Catalog Number: 759709
Pricing

Send a RFQ.

View RFQ List
Category
Cell Cycle Reagents
TagsReagentsFlow Cytometry Reagentsresearch-reagents
CollectionsBD Flow Cytometry Reagents for Multicolor PanelsBD Single-Cell Multiomics Reagents for the BD Rhapsody™ PlatformBD Immunoassay Reagents: CBA, ELISA & ELISPOT for Cytokine Panels
Manufacturer's Pagewww.bdbiosciences.com/en-us/products/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/ry610-rat-anti-mouse-enpp1.759709
Description

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
3. For U.S. patents that may apply, see bd.com/patents.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
7. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
9. An isotype control should be used at the same concentration as the antibody of interest.
10. CF™ is a trademark of Biotium, Inc.
Specifications

General

SourceHuman BDNF, amino acids His129-Arg247 (Accession# P23560) was expressed in CHO cells.
Molecular MassThe 119 amino acid recombinant protein has a predicted molecular mass of approximately 13.5 kD. The DTT-reduced and non-reduced proteins migrate at approximately 14.5 kD by SDS-PAGE. The predicted N-terminal amino acid is His.
Purity>95%, as determined by Coomassie stained SDS-PAGE.
FormulationLyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & HandlingUnopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.
ApplicationELISA - Quality tested
Recommended UsageEach lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 156.3 to 10,000 pg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application NotesThis human BDNF protein is useful as a standard for a human BDNF sandwich ELISA, using unlabeled A15062G antibody (Cat. No. 536702) as capture and biotinylated A15062F antibody (Cat. No. 536804) as detection.
StructureNoncovalently linked homodimer
DistributionUbiquitous with high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung, and testis.
FunctionSupports the survival of sensory and sympathetic neurons, and also dopaminergic midbrain neurons.
InteractionSensory neurons, sympathetic neurons, midbrain dopaminergic neurons.
Ligand/ReceptorTrkB
Biology AreaCell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular FamilyCytokines/Chemokines, Growth Factors
Antigen ReferencesBaloh RH, et al. 1998. Neuron. 21:1291 Masure S, et al. 1999. Eur. J. Biochem. 266:892-902. Gardell LR, et al. 2003. Nat. Med. 9(11):1383-9. Honma Y, et al. 2002. Neuron. 35:267-82. Banerjee A, et al. 2012. PLoS One 7(11):e50098. Wong LE, et al. 2015. Proc. Natl. Acad. Sci. USA 112:6170-5. Zhang M, et al. 2016. Oncotarget. 7:3267-82.
Gene ID627
UniProtView information about BDNF on UniProt.org
Regulatory StatusRUO
Other NamesBrain-derived neurotrophic factor, ANON2, BLUN2