| Source | Human CD200R1, amino acids Ser24-Leu266 (Accession # AAQ19772) with a C-terminal human IgG1 Fc and 6-His tag was expressed in 293E cells. |
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| Molecular Mass | The 464 amino acid recombinant protein has a predicted molecular mass of approximately 52.2 kD. The DTT-reduced protein migrates at approximately 75 kD and and non-reduced protein migrates at approximately 150 kD by SDS-PAGE. The predicted N-terminal amino acid is Ser. |
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| Purity | >95%, as determined by Coomassie stained SDS-PAGE. |
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| Formulation | 0.22 µm filtered protein solution is in PBS, pH 7.2 |
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| Endotoxin Level | Less than 0.1 EU per µg protein as determined by the LAL method. |
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| Concentration | 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
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| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | When human CD200R1 is immobilized at 0.5 µg/mL, biotinylated human CD200 binds with EC50 of 1.5 – 6.0 ng/mL. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Disulfide-linked homodimer |
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| Distribution | Mast cells, basophils, macrophages, and dendritic cells |
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| Function | Inhibitory receptor for the CD200. Limits inflammation by inhibiting the expression of proinflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. |
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| Interaction | Neurons and vascular endothelium in CNS, and thymocytes, T and B cells, and dendritic cells. |
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| Ligand/Receptor | CD200 |
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| Bioactivity | Measured in a competition binding assay with biotinylated human CD200. |
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| Biology Area | Immunology, Costimulatory Molecules, Inhibitory Molecules |
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| Molecular Family | CD Molecules, Soluble Receptors |
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| Antigen References | 1. Hernangomez M, C. et al. 2014. Curr Pharm Des. 20:4707-22. 2. Walker DG, Lue LF. 2013. Future Neurol. 10.2217/fnl.13.14. 3. Wright GJ, et al. 2003. J Immunol. 171: 3034-46. 4. Vaine CA, Soberman RJ. 2014. Adv Immunol. 121:191-211. 5. Minas K, et al. 2006. Crit Rev Immunol. 26(3): 213-30. 6. Wang XJ, et al. 2007. J Neuroimmune Pharmacol. 2(3): 259-64. 7. Wong KK, et al. 2010. J Leukoc Biol. 88(2): 361-72. |
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| Gene ID | 131450 |
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| UniProt | View information about CD200R1 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | CD200 receptor 1, CD200R, CRTR2, MOX2 receptor, MOX2R, OX2 receptor, OX-2 receptor, OX2R |
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