BD, 774502, BV605 Mouse Anti-Human HLA-DRB1 NFLD.D2

Name: BV605 Mouse Anti-Human HLA-DRB1 NFLD.D2
Brand: BD
SKU: 774502

50 µg

Catalog Number: 774502

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Category

Cell Cycle Reagents

TagsReagents Flow Cytometry Reagents research-reagents

CollectionsBD Flow Cytometry Reagents for Multicolor Panels BD Single-Cell Multiomics Reagents for the BD Rhapsody™ Platform BD Immunoassay Reagents: CBA, ELISA & ELISPOT for Cytokine Panels

Manufacturer's Pagewww.bdbiosciences.com/en-us/products/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/bv605-mouse-anti-human-hla-drb1.774502

Description

The NFLD.D2 monoclonal antibody specifically recognizes polymorphic epitopes on the HLA class II histocompatibility antigen, DRB1 (HLA-DRB1). These include HLA-DR molecules carrying the HLA-DRB1:70-74:QKRAA/QRRAA sequence (DRB1*0401, 0404, 0405, 0408, 0409, 0410, 0413, 0416, 0419, 0101, 0102, 1402, 1406, or 1409) whose expression is implicated with certain diseases including rheumatoid arthritis. The NFLD.D2 antibody also recognizes HLA-DRB5:70-74:QARAA (DRB1*1601, 1602, 1604; DRB5*0201,0202) and can weakly bind to HLA-DRB1:70-74:QRRAE (DRB1*0403, 0406, 0407, 0411). HLA-DR antigens are MHC Class II heterodimers comprised of type I transmembrane glycoprotein alpha and beta subunits that contain two extracellular domains, transmembrane regions, and cytoplasmic tails. The ~34 kDa HLA-DR alpha (HLA-DRα) chain is encoded by HLA-DRA whereas the alternative ~28 kDa HLA-DR beta (HLA-DRβ) chains are encoded by one of the four different HLA-DRB loci (HLA-DRB1,3,4, and 5) that are located within the Human Leukocyte Antigen (HLA) Complex of chromosome 6. HLA- DRB1 is variably expressed on antigen-presenting cells (APCs), such as dendritic cells, B lymphocytes, monocytes, macrophages, and thymic epithelial cells as well as by activated T cells. These typically display peptide antigens peptides on professional antigen presenting cells (APCs) for recognition by CD4+ TCR αβ T cells that are HLA-DRB1-restricted.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application. For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
3. For U.S. patents that may apply, see bd.com/patents.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
8. An isotype control should be used at the same concentration as the antibody of interest.
9. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from BD Horizon™ BV421 may be observed. Therefore, we recommend that individual compensation controls be performed for every BD Horizon™ BV605 conjugate.
10. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
11. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.

Specifications

General

Source	Mouse IL-22Rα1, amino acid (Thr18-Ala228) (Accession: # Q80XZ4.1), with a C-terminal 6His tag, was expressed in 293E cells.
Molecular Mass	The 217 amino acid recombinant protein has a predicted molecular mass of approximately 24.89 kD. The DTT-reduced and non-reduced protein migrates at approximately 36 kD by SDS-PAGE. The predicted N-terminal amino acid is Thr.
Purity	>98%, as determined by Coomassie stained SDS-PAGE.
Formulation	0.22 µm filtered protein solution is in 1x PBS.
Endotoxin Level	Less than 0.1 EU/µg cytokine as determined by the LAL method.
Concentration	10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.