The 203E3 monoclonal antibody specifically recognizes Integrin alpha 11 (Integrin α11), a ~155 kDa type I transmembrane glycoprotein encoded by the ITGA11 gene. Integrin α11 preferentially associates with the Integrin beta 1 chain (CD29) in a noncovalent manner to form the Integrin α11β1 heterodimer. This heterodimer functions as an adhesion receptor for various collagens, including type I collagen. Integrin α11 is most highly expressed by embryonic fibroblasts, myofibroblasts, mesenchymal stem cells and smooth muscle cells and is involved in collagen remodeling which is involved in the development, functioning, and repair of tissues. In certain human tumors, such as non-small cell lung carcinomas (NSCLC) and head and neck squamous cell carcinoma (HNSCC), cells—particularly activated stromal cells—can express elevated levels of Integrin α11, which is associated with increased tumorigenicity. The overexpression of Integrin α11 by cancer-associated fibroblasts (CAFs) may in part be regulated by the presence of TGFβ activity in the tumor microenvironment. Additionally, myofibroblasts overexpressing integrin α11 may be linked to fibrotic disease.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
3. For U.S. patents that may apply, see bd.com/patents.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
8. An isotype control should be used at the same concentration as the antibody of interest.
9. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email:
[email protected].
10. Alexa Fluor™ is a trademark of Life Technologies Corporation.
11. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
