Description
The CC8-75 monoclonal antibody specifically recognizes B Cell Maturation Antigen (BCMA) which is also known as B Cell Maturation Protein or CD269. CD269 (BCMA) is an ~20 kDa type III transmembrane protein that is encoded by Tnfrsf17 (tumor necrosis factor receptor superfamily, member 17). This protein is expressed as a homotrimer on the surface of germinal center B cells, plasmablasts, and plasma cells and may be highly expressed on some other B lineage cell lines, eg, myeloma cells. CD269 (BCMA) functions as a receptor for CD256 (APRIL) and CD257 (BAFF) homotrimers that are involved in driving the proliferation, differentiation and survival of B cells into plasma cells. As in the human system, these TNF superfamily ligands signal through CD269 (BCMA) which makes this receptor a key target in mouse models for human diseases like multiple myeloma. A soluble form of CD269 (BCMA) is generated by γ-secretase that can cleave the transmembrane form of this receptor from cell surfaces. This soluble form may serve as a decoy receptor by binding to ligands and limiting their availability for ligand bound receptor-mediated signaling.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application. For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
3. For U.S. patents that may apply, see bd.com/patents.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
8. An isotype control should be used at the same concentration as the antibody of interest.
