Description
The W3/25 monoclonal antibody specifically recognizes rat CD4, a type I transmembrane glycoprotein of ~ 55 kDa encoded by the Cd4 gene which belongs to the immunoglobulin superfamily. CD4 is variably expressed on most thymocytes, a subset of peripheral mature T lymphocytes, monocytes, macrophages, as well as some dendritic cells and microglia. CD4 functions as an antigen coreceptor on developing and mature T cells, binding to nonpolymorphic regions of MHC class II molecules expressed on antigen-presenting cells such as dendritic cells, macrophages, and B cells. This interaction is crucial for CD4-associated T-cell antigen receptor (TCR)-linked intracellular signal transduction pathways involving the protein tyrosine kinase Lck. While both W3/25 and OX-38 antibodies bind to identical or adjacent epitopes on CD4, the W3/25 antibody binds to a different epitope than the OX-35 monoclonal antibody does. The W3/25 antibody can reportedly inhibit CD4 coreceptor functions as shown in vitro and in vivo model systems including autoimmune diseases.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
3. For U.S. patents that may apply, see bd.com/patents.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
8. An isotype control should be used at the same concentration as the antibody of interest.
9. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
