Description
The NZ-1 monoclonal antibody specifically binds with high affinity to Podoplanin. This antibody was generated by immunizing mice with a 14-residue peptide segment (aa 38-51) from a platelet aggregation-stimulating (PLAG) domain of human Podoplanin. For this reason, this antibody is useful to detect the Podoplanin molecule as well as target proteins, such as antibodies that are labeled with a dodecapeptide (GVAMPGAEDDVV) named PA Tag. Podoplanin is a ~43 kDa mucin-type 1 transmembrane glycoprotein composed of a serine- and threonine-rich intracellular domain, a single transmembrane domain and a short intracellular domain. This multifunctional glycoprotein is expressed on various cell types, including intestinal and thymic epithelial cells, lymphatic endothelial cells, kidney podocytes, alveolar type I cells, fibroblastic reticular cells, macrophages, Th17 cells and osteocytes. Podoplanin expression is upregulated in inflammation and cancer. The binding of Podoplanin to the C-type lectin domain family 1 member B (Clec1B, also known as CLEC-2) on the surface of platelets results in platelet aggregation/activation, thrombosis, lymphatic vessel development, and cancer invasion and metastasis. The Podoplanin (PA Tag) antibody can be used for detecting tagged target proteins in cells that do not express endogenous Podoplanin. This antibody specifically reacts with the human Podoplanin sequence and does not react with Podoplanin sequences from mouse, rat, hamster or dog. Reportedly, this antibody can be used for Western blot, immunohistochemistry, immunoprecipitation or imaging applications.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application. For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
3. For U.S. patents that may apply, see bd.com/patents.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
8. An isotype control should be used at the same concentration as the antibody of interest.
9. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from BD Horizon™ BV421 may be observed. Therefore, we recommend that individual compensation controls be performed for every BD Horizon™ BV605 conjugate.
10. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
11. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
