Description
The S5/1 monoclonal antibody specifically recognizes CD88 which is also known as C5a receptor (C5aR), Complement C5a receptor 1 (C5AR1) or C5a anaphylatoxin chemotactic receptor 1. CD88 (C5aR) is an ~43 kDa G type protein-coupled receptor with seven transmembrane domains encoded by C5AR1 (complement C5a receptor 1) which belongs to the rhodopsin superfamily. This receptor is variably expressed on neutrophils, basophils, eosinophils, monocytes, macrophages, dendritic cells, mast cells as well as human cell lines of myeloid origin, and certain cell types in several organs, including liver hepatocytes, lung bronchial and alveolar epithelial cells, lung vascular smooth muscle, endothelial cells, astrocytes, microglia, and fibroblast-like cells of the brain. The binding of the anaphylatoxin ligands C5a or C5a(desArg) to CD88 (C5aR) leads to various cellular responses including the shedding of selectins, upregulation of adhesion molecules, chemotaxis, granule exocytosis, activation of NADPH oxidase, and superoxide anion production. The S5/1 monoclonal antibody reportedly blocks anaphylatoxin ligand binding to CD88 (C5aR).
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
3. For U.S. patents that may apply, see bd.com/patents.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
8. An isotype control should be used at the same concentration as the antibody of interest.
9. CF™ is a trademark of Biotium, Inc.
10. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
