Description
The 6B7 monoclonal antibody specifically binds to IL-17 Receptor B (IL-17RB), which is a single-pass type I transmembrane protein and a component of the heterodimeric receptor for IL-25 (also known as IL-17E). Several components of the IL-17RB signaling mechanism have been identified, including ACT1, TRAF4, SMURF2, DAZAP2, and STAT5. The cytoplasmic tails of both of the IL-25 Receptor components, IL-17RA and IL-17RB, and ACT1 contain the SEFIR (similar expression to fibroblast growth factor genes and IL-17R) domain that is essential for IL-25R-mediated signaling. Signaling through IL-17RB mediates Th2 immune responses and is responsible for pathogenesis in a murine model for asthma. IL-17RB is found on subsets of iNKT cells, Th2 cells, some myeloid cells in solid organs, and type 2 innate lymphoid cells (ILC2) in lung, peritoneum, mesenteric lymph nodes and bone marrow.
Put all BD® AbSeq Reagents to be pooled into a Latch Rack for 500 µL Tubes (Thermo Fisher Scientific Cat. No. 4900). Arrange the tubes so that they can be easily uncapped and re-capped with an 8-Channel Screw Cap Tube Capper (Thermo Fisher Scientific Cat. No. 4105MAT) and the reagents aliquoted with a multi-channel pipette. BD® AbSeq tubes should be centrifuged for ≥ 30 seconds at 400 × g to ensure removal of any content in the cap/tube threads prior to the first opening.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography and conjugated to BD® AbSeq oligonucleotide under optimal conditions.
1. This reagent has been pre-diluted for use at the recommended volume per test. Typical use is 2 µl for 1 × 10^6 cells in a 200-µl staining reaction.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
5. Illumina is a trademark of Illumina, Inc.
6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
7. Please refer to bd.com/genomics-resources for technical protocols.
8. For U.S. patents that may apply, see bd.com/patents.
