Bio-Rad, MCA6400A488, CD94 antibody | 8H10

Mouse anti Dog CD94 antibody, clone 8H10 recognizes canine CD94, also known as KLRD1 (Killer cell lectin like receptor D1). CD94 is expressed on natural killer (NK) cells and on natural killer-like T (NKT) cells. In humans, a range of receptors that can identify and modulate NK cell function have been well described. Among them CD94 which associates with members from the NKG2 family to form activating or inhibitory heterodimers. It is not established whether the same interaction happens in dogs. Canine NK cells remain poorly characterized with mouse anti dog CD94 being one of few markers available to detect them ( Graves et al . 2019 ). Mouse anti dog CD94 antibody, clone 8H10 will detect approx. 7.7% (NK cell range in dogs: 2.5% – 15%) (
Graves et al . 2019 , Kisseberth and Lee, 2021 ) of freshly isolated canine PBMC and has been used for magnetic bead cell isolation ( Graves et al . 2019 ).

Storage Information
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.