Description
Recombinant Human MMP-3 Matrix metalloproteinase-3 (MMP-3), also known as stromelysin-1 (SL-1) and transin-1 is a member of the metzincins superfamily of proteases and is produced by various cells and tissues including the endothelium, vascular smooth muscle cells intima and platelets ( Cui et al. 2017 ). MMP-3 is largely expressed by connective and vascular tissues and cells due to its roles in extracellular matrix remodeling. It is synthesized as a pre-proenzyme before being processed to remove the signal peptide forming the 57 kDa pro-MMP-3. This is then further cleaved by proteolytic enzymes to create the 45 kDa active form. Like other MMPs, MMP-3 plays a major role in tissue remodeling through the promotion of extracellular matrix (ECM) protein turnover. Specifically MMP-3 degrades the collagen proteins, type II, IV, IX as well as the proteoglycans elastin fibronectin and laminin ( Cui et al. 2017 ). Through the degradation of these ECM components, growth factors and adhesion molecules, MMP-3 can modify the extracellular environment and modulate cell to cell signaling. As such, MMP-3 is implicated in synaptic plasticity through enzymatic modification of ECM constituents ( Lech et al. 2019 ).
Storage Information
Prior to reconstitution store at -70°C. Following reconstitution store at -80°C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
