Biolegend, 100613, Alexa Fluor® 647 anti-mouse CD5 Antibody
25microg
Catalog Number: 100613
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Mouse thymus or spleen |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Red Laser (633 nm) |
| Application Notes |
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| Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections. |
| Application References(PubMed link indicates BioLegend citation) | Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP) Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC) Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. |
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| Product Citations | Kaku H, et al. 2014. J Immunol. 193:5904. PubMed Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed Rosser EC, et al. 2020. Cell Metabolism. 31(4):837-851. PubMed Smith LK, et al. 2021. Elife. 10:. PubMed |
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| RRID | AB_493168 (BioLegend Cat. No. 100613) AB_2075301 (BioLegend Cat. No. 100614) |
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| Structure | Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD |
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| Distribution | Thymocytes, T cells, B-1 cells |
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| Function | Negative regulator of T-B cell interaction |
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| Ligand/Receptor | CD72, gp35-37 |
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| Cell Type | B cells, T cells, Thymocytes |
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| Biology Area | Costimulatory Molecules, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kipps TJ. 1988. Adv. Immunol. 47:117. 3. Antin JH, et al. 1985. J. Immunol. 136:505. 4. Tarakhovsky A, et al. 1995. Science 269:535. |
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| Gene ID | 12507 |
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| UniProt | View information about CD5 on UniProt.org |
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| Clone | 53-7.3 |
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| Regulatory Status | RUO |
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| Other Names | Lyt-1, Ly-1, T1, Tp67, Ly-12 |
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| Isotype | Rat IgG2a, κ |
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