Biolegend, 108317, TotalSeq™-C1034 anti-mouse Ly-51 Antibody
10microg
Catalog Number: 108317
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | C57BL/6 mouse Pre-B lymphoma cell line (L1-2) plus Abelson murine leukemia virus-specific cytotoxic T-cell clones |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA |
| Preparation | The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | PG - Quality tested |
| Recommended Usage |
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| Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution. To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions. Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. |
| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation1,2 and immunohistochemical staining3-5, 7-8 of acetone-fixed frozen sections. |
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| Additional Product Notes | TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq. The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation. View more applications data for this product in our Scientific Poster Library. |
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| Application References(PubMed link indicates BioLegend citation) | Pillemer E, et al. 1984. P. Natl. Acad. Sci. USA 81:4434. (IP) Hardy RR, et al. 1991. J. Exp. Med. 173:1213. (IP) Adkins B, et al. 1988. Immunogenetics 27:180. (IHC) Surh CD, et al. 1992. J. Exp. Med. 176:611. (IHC) Goverman J, et al. 1997. Immunol. Today 18:204. (IHC) Dumont-Lagace M, 2014. J. Immunol. 192:2219. PubMed Kvell K, et al. 2010. PLoS One 5:e10701. (FC, IHC) Griewank K, et al. 2007. Immunity 27:751. (FC, IHC) |
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| RRID | AB_2860617 (BioLegend Cat. No. 108317) |
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| Structure | Aminopeptidase A, 140 kD |
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| Distribution | B progenitors, bone marrow stromal lines, thymic dendritic cells, cortical epithelial cells |
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| Function | Aminopeptidase, may function in IL-7-driven proliferation of B cell precursors |
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| Cell Type | B cells, Dendritic cells, Epithelial cells |
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| Biology Area | Immunology |
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| Antigen References | 1. Goverman J, et al. 1997. Immunol. Today 18:204. 2. Morse HC, et al. 1987. J. Exp. Med. 165:920. 3. Sherwood P, et al. 1990. Int. Immunol. 2:399. 4. Lin Q, et al. 1998. J. Immunol. 160:4681. |
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| Gene ID | 13809 |
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| UniProt | View information about Ly-51 on UniProt.org |
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| Clone | 6C3 |
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| Regulatory Status | RUO |
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| Other Names | 6C3/BP-1 |
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| Isotype | Rat IgG2a, κ |
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| Barcode Sequence | TCCGTTTGTTGTTGT |
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