Biolegend, 260548, GMP APC anti-human IgM Antibody
100tests
Catalog Number: 260548
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Manufacturer's Pagewww.biolegend.com/en-us/products/gmp-apc-anti-human-igm-antibody-27488
| Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human Ig cocktail |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer |
| Preparation | The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. |
| Concentration | 50 µg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Excitation Laser | Red Laser (633 nm) |
| Application Notes |
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| MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis. Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended. |
| Application References(PubMed link indicates BioLegend citation) | Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed |
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| Disclaimer | GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products |
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| Structure | Ig family |
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| Distribution | B cells |
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| Function | B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis |
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| Cell Type | B cells |
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| Biology Area | Immunology |
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| Gene ID | 3507 |
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| UniProt | View information about IgM on UniProt.org |
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| Clone | MHM-88 |
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| Other Names | Immunoglobulin M |
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| Isotype | Mouse IgG1, κ |
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