Biolegend, 285168, Spark Blue™ 574 anti-human CD62L (Flexi-Fluor™) Antibody

Name: Spark Blue™ 574 anti-human CD62L (Flexi-Fluor™) Antibody
Brand: Biolegend
SKU: 285168

50microg

Catalog Number: 285168

Pricing

Request a quote.

View RFQ List

Category

General Purpose Solvents

Pack Size62 L

Manufacturer's Pagewww.biolegend.com/en-us/products/spark-blue-574-anti-human-cd62l-flexi-fluor-antibody-25806

Description

CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.

Specifications

General

Verified Reactivity	Human
Reported Reactivity	Chimpanzee, Cow
Antibody Type	Monoclonal
Host Species	Mouse
Immunogen	Concentrated supernatant from PMA-activated human peripheral blood leukocytes
Formulation	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation	The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
Concentration	0.2 mg/mL
Storage & Handling	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application	FC
Recommended Usage	Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. * Spark Blue™ 574 has a maximum excitation of 506 nm and a maximum emission of 574 nm.